Here, we will share the hiPSC & organoid protocols & recipes currently in use at the hub.

This is a living document; we will share the latest versions of the recipes and protocols as the hub optimizes and improves upon their current iterations.

Recipes

Patterning recipes to generate
human Cortical Spheroids (hCS),
human Subpallial Spheroids (hSS),
human Spinal Cord Spheroids (hSpS).
Up-to-date as of November 2023.

Protocols

Videos

Protocol videos prepared by the hub members!

hiPSC passaging (feat. Zihui)

Brain Organoid Formation (Day 0):
Dislodging Aggregated hiPSCs (feat. Zihui)

Brain Organoid Formation (Day -1):
Microwell aggregation of hiPSCs (feat. Zihui)

Recipes & Protocols